Human U1 RNA genes contain an unusually sensitive nuclease S1 cleavage site within the conserved 3' flanking region.

Heteroduplex cleavage analysis using S1 nuclease.

A variety of methods have been developed to screen for genetic mutations. Most rely on the comparative analysis of polymerase chain reaction (PCR) products. Single-strand conformational polymorphism (4), the most widely used technique, relies on sequence-dependent conformational electrophoretic mobility differences between wild-type (WT) and mutant strands; whereas, heteroduplex analysis (5,8),...

Elongation factor G protects a nuclease-sensitive site of 23 S RNA within the ribosome.

Site-specific DNA cleavage within the MLL breakpoint cluster region

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Accurate transcription of cloned Xenopus rRNA genes by RNA polymerase I: demonstration by S1 nuclease mapping.

We have demonstrated faithful transcriptional initiation of cloned Xenopus rRNA genes upon injection into Xenopus oocytes. This observation has been made possible by the use of an S1 nuclease assay that is both sensitive and quantitative. In order to detect rRNA synthesis from the injected template above the large background of rRNA endogenously present in oocytes, the divergence of ribosomal D...

Specific hydrolysis of rabbit globin messenger RNA by S1 nuclease.

S1 nuclease isolated from Aspergillus oryzae has been used to investigate the secondary structure of rabbit globin messenger RNA (mRNA). The enzyme, which is specific for single stranded nucleotides, digests globin mRNA to a limited extent, with 65-75% of the mRNA nucleotides resistant to digestion under mild conditions. This limited digestion is not due to enzyme inactivation, but rather to th...